Insulin metabolism in erythrocytes and hepatocytes of normal and experimental diabetic rats was studied. Insulin-degrading complex of erythrocytes and hepatocytes was investigated at low insulin concentration in blood. Kinetic parameters of insulinase depend on insulin concentration in blood have shown. Thermo-acid resistant non-competitive endogenous inhibitors were purified from erythrocytes and hepatocytes. Increase of endogenous inhibitors influence insulin-degrading enzyme in diabetes. Synthesis of insulin-degrading enzyme in hepatocytes of diabetic rats was reduced. These results suggest that insulin-degrading activity in erythrocytes and hepatocytes is regulated by endogenous inhibitors, by synthesis of insulinase. This change of blood insulin concentration influences practically all the components of insulin-degrading complex.
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Nikolaev S.L., Strelkova M.A., Komov V.P. (2001) Insulin degradation in hepatocytes and erythrocytes from normal and experimental diabetic rats. Voprosy Meditsinskoi Khimii, 47(3), 329-337.
Nikolaev S.L. et al. Insulin degradation in hepatocytes and erythrocytes from normal and experimental diabetic rats // Voprosy Meditsinskoi Khimii. - 2001. - V. 47. -N 3. - P. 329-337.
Nikolaev S.L. et al., "Insulin degradation in hepatocytes and erythrocytes from normal and experimental diabetic rats." Voprosy Meditsinskoi Khimii 47.3 (2001): 329-337.
Nikolaev, S. L., Strelkova, M. A., Komov, V. P. (2001). Insulin degradation in hepatocytes and erythrocytes from normal and experimental diabetic rats. Voprosy Meditsinskoi Khimii, 47(3), 329-337.
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