The influence of platinum derivatives, cisplatin (cis-diamminedichloroplatinum) and imidazolplatin (cis-diimidazoledichloroplatinum) on the activity of rat liver microsomal NADPH-oxidoreductases was investigated in vitro using spectrophotometrical and chemiluminometrical methods. In the range of concentrations from 1 to 12 µM cisplatin inhibited NADPH-cytochrome c-reductase activity and NADPH/lucigenin-dependent chemiluminescence of microsomes. The 3 µM cisplatin decreased the NADPH-dependent reductase activity by 50%. At concentrations less than 2,5 mM sanazole (drug AK-2123) did not prevent the inhibiting influence of cisplatin on microsomal NADPH-dependent reductases. Imidazolplatin insignificantly inhibited NADPH-reductases. It is concluded that negligible inhibiting effect of imidazolplatin on microsomal NADPH-oxidoreductase allows us to consider this platinum derivative as a promising compound for further experimental trials as anticancer drug with low toxic action on the normal tissues of an organism.
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Shchepetkin I.A., Plotnikov V.M., Kagiya V.T. (2001) Platinum derivative effect on activity of microsomal NADPH-oxidoreductases. Voprosy Meditsinskoi Khimii, 47(4), 373-381.
Shchepetkin I.A. et al. Platinum derivative effect on activity of microsomal NADPH-oxidoreductases // Voprosy Meditsinskoi Khimii. - 2001. - V. 47. -N 4. - P. 373-381.
Shchepetkin I.A. et al., "Platinum derivative effect on activity of microsomal NADPH-oxidoreductases." Voprosy Meditsinskoi Khimii 47.4 (2001): 373-381.
Shchepetkin, I. A., Plotnikov, V. M., Kagiya, V. T. (2001). Platinum derivative effect on activity of microsomal NADPH-oxidoreductases. Voprosy Meditsinskoi Khimii, 47(4), 373-381.
