VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Platinum derivative effect on activity of microsomal NADPH-oxidoreductases

   
Shchepetkin I.A.1, Plotnikov V.M.1, Kagiya V.T.2

1. Institute of Oncology, Tomsk Research Center, Siberian Branch of Russian Academy of Medical Sciences
2. Health Research Foundation, Kyoto
PubMed Id: 11693025
Year: 2001  Volume: 47  Issue: 4  Pages: 373-381
The influence of platinum derivatives, cisplatin (cis-diamminedichloroplatinum) and imidazolplatin (cis-diimidazoledichloroplatinum) on the activity of rat liver microsomal NADPH-oxidoreductases was investigated in vitro using spectrophotometrical and chemiluminometrical methods. In the range of concentrations from 1 to 12 µM cisplatin inhibited NADPH-cytochrome c-reductase activity and NADPH/lucigenin-dependent chemiluminescence of microsomes. The 3 µM cisplatin decreased the NADPH-dependent reductase activity by 50%. At concentrations less than 2,5 mM sanazole (drug AK-2123) did not prevent the inhibiting influence of cisplatin on microsomal NADPH-dependent reductases. Imidazolplatin insignificantly inhibited NADPH-reductases. It is concluded that negligible inhibiting effect of imidazolplatin on microsomal NADPH-oxidoreductase allows us to consider this platinum derivative as a promising compound for further experimental trials as anticancer drug with low toxic action on the normal tissues of an organism.
Download PDF:
Reference:

Shchepetkin I.A., Plotnikov V.M., Kagiya V.T. (2001) Voprosy meditsinskoi khimii, 47(4), 373-381.
References  
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
        

© Institute of Biomedical Chemistry, Moscow, Russia