Platinum derivative effect on activity of microsomal NADPH-oxidoreductases

Shchepetkin I.A.1, Plotnikov V.M.1, Kagiya V.T.2

1. Institute of Oncology, Tomsk Research Center, Siberian Branch of Russian Academy of Medical Sciences
2. Health Research Foundation, Kyoto
PubMed Id: 11693025
Year: 2001  Volume: 47  Issue: 4  Pages: 373-381
The influence of platinum derivatives, cisplatin (cis-diamminedichloroplatinum) and imidazolplatin (cis-diimidazoledichloroplatinum) on the activity of rat liver microsomal NADPH-oxidoreductases was investigated in vitro using spectrophotometrical and chemiluminometrical methods. In the range of concentrations from 1 to 12 µM cisplatin inhibited NADPH-cytochrome c-reductase activity and NADPH/lucigenin-dependent chemiluminescence of microsomes. The 3 µM cisplatin decreased the NADPH-dependent reductase activity by 50%. At concentrations less than 2,5 mM sanazole (drug AK-2123) did not prevent the inhibiting influence of cisplatin on microsomal NADPH-dependent reductases. Imidazolplatin insignificantly inhibited NADPH-reductases. It is concluded that negligible inhibiting effect of imidazolplatin on microsomal NADPH-oxidoreductase allows us to consider this platinum derivative as a promising compound for further experimental trials as anticancer drug with low toxic action on the normal tissues of an organism.
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Shchepetkin I.A., Plotnikov V.M., Kagiya V.T. (2001) Voprosy meditsinskoi khimii, 47(4), 373-381.
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