Determination of low protein concentration in biological fluids. Methodological aspects. (usige of experience of matematical analysis)
Altchouler B.Y.1, Rakov S.S.2, Tkachev G.A.3
1. Bauman Clinical Hospital №29 2. Russian Academy of Postgraduate Education, Chair of Clinical Laboratory Diagnostics 3. Moscaw State University of Nature Development, Chair of Higher Mathematics
We investigated the effect of albumin/globylin ratio on the analytic error of protein determination. Several methods were used: Bradford, Lowry, pyrogallol-red, biuret, and Sulfosalicylic acid. Reagents used in Lowry method exhibit higher affinity to globulins, whereas reagents used in other methods have higher affinity to albumins. Although the biuret method demonstrated equal efficiency in determining concentrations of various proteins low sensitivity of this method limits its clinical applicability. For other methods there was non-linear dependence of analytic error on protein composition in the sample. Combination of two methods for protein determination (using one calibrator) completely removes analytic error.
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Altchouler B.Y., Rakov S.S., Tkachev G.A. (2001) Determination of low protein concentration in biological fluids. Methodological aspects. (usige of experience of matematical analysis). Voprosy Meditsinskoi Khimii, 47(4), 426-438.
Altchouler B.Y. et al. Determination of low protein concentration in biological fluids. Methodological aspects. (usige of experience of matematical analysis) // Voprosy Meditsinskoi Khimii. - 2001. - V. 47. -N 4. - P. 426-438.
Altchouler B.Y. et al., "Determination of low protein concentration in biological fluids. Methodological aspects. (usige of experience of matematical analysis)." Voprosy Meditsinskoi Khimii 47.4 (2001): 426-438.
Altchouler, B. Y., Rakov, S. S., Tkachev, G. A. (2001). Determination of low protein concentration in biological fluids. Methodological aspects. (usige of experience of matematical analysis). Voprosy Meditsinskoi Khimii, 47(4), 426-438.
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