The stability of collagen molecules and moisture capacity of human normal and osteoarthrotic (ОА) cartilage were studied before and after extraction of glycosaminoglycans (GAG) by 4M guanidinum chloride. The content and nature of water were determined by Fisher titration, DSC and analysis of sorbtion-desorbtion processes of water vapour in cartilage. The stability of collagen molecules was determined by the degree of enzymatic hydrolysis: collagenase, pronase and pepsin. It was found that weakenig of bonds between main compounds of the cartilage matrix and decrease of GAG quantities in the OA cartilage were accompanied by structural disorganization of the collagen network, which is manifested by breakdowns of intramolecular bonds in telopeptides and intermolecular bonds in the spiral part of collagen molecules, these changes may contribute to increase of total water in OA cartilage. The correlation of free and bound water fractions in cartilage was increased from 5 to 44 in OA cartilage. These results can be used as a criterion of pathological condition of human articular cartilage
Kim Zong Chhol et al. The changes of biochemical characteristics of collagen and nature of water in human osteoarthrotic cartilage // Voprosy meditsinskoi khimii. - 2001. - V. 47. -N 5. - P. 498-505.
Kim Zong Chhol et al., "The changes of biochemical characteristics of collagen and nature of water in human osteoarthrotic cartilage." Voprosy meditsinskoi khimii 47.5 (2001): 498-505.
Kim, Zong, Chhol,, Bykov, V. A., Nikolaeva, S. S., Rebrova, G. A., Roshina, A. A., Yakovleva, L. B., Korolyova, O. A., Rebrov, L. B. (2001). The changes of biochemical characteristics of collagen and nature of water in human osteoarthrotic cartilage. Voprosy meditsinskoi khimii, 47(5), 498-505.
Павлова В.Н., Копьева Т.Н., Слуцкий Л.И., Павлов Г.Г. (1988) Хрящ, М., Медицина, 320. Scholar google search
BankRund A., Bayliss Mitcheal Т., Lafeber Floris R.J.G., Maroudas Alice (1998) Biochem. J., 330 (1), 345- 351. Scholar google search