Human cytochrome Р450 2В6 gene from plasmid pUC9, carrying cytochrome CYP2B6 cDNA was cloned into eucariotic expression vector pcDNA 3.1 (+). Cytochrome P450 2B6 gene in recombinant plasmid pcDNA 3.1 (+) /СУР 2В6 was expressed in E.coli cells. The expression of catalytically active recombinant protein was 60-100 nm per liter of the culture medium
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Kolomeichuk S.N., Pokrovskaya M.V., Borisova A.A., Jgoun A.A., Solodar L.I., Sokolov N.N., Shvets V.I., Gervaziev Yu.V., Eldarov M.A., Archakov A.I. (2001) Cloning of the human cytochrome P450 2B6 gene in Escherichia coli cells. Voprosy Meditsinskoi Khimii, 47(6), 609-619.
Kolomeichuk S.N. et al. Cloning of the human cytochrome P450 2B6 gene in Escherichia coli cells // Voprosy Meditsinskoi Khimii. - 2001. - V. 47. -N 6. - P. 609-619.
Kolomeichuk S.N. et al., "Cloning of the human cytochrome P450 2B6 gene in Escherichia coli cells." Voprosy Meditsinskoi Khimii 47.6 (2001): 609-619.
Kolomeichuk, S. N., Pokrovskaya, M. V., Borisova, A. A., Jgoun, A. A., Solodar, L. I., Sokolov, N. N., Shvets, V. I., Gervaziev, Yu. V., Eldarov, M. A., Archakov, A. I. (2001). Cloning of the human cytochrome P450 2B6 gene in Escherichia coli cells. Voprosy Meditsinskoi Khimii, 47(6), 609-619.
Chang Т.К., Weber G.F., Crespi C.L., Waxman D.J. (1993). Cancer Res., 53, 5629- 5637. Scholar google search
Code E.L., Crespi C.L, Penman B.W. et al. (1997). Drug Metab. Dispos., 25, 985- 993. Scholar google search
Sambrook J.F., Fritsch E.F., Maniatis T. (1989). Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. Scholar google search
Birnboim H.C., Doly J. (1979) Nucleic Acids Res., 7,1513- 1523. Scholar google search
