The synthesized decapeptide represents functionally important site for binding to the insulin receptor. Amino acid residues at position, 1-8 correlate with B-chain of insulin at position B19-B26, and the residues at position 9-10 correlate with A-chain at position A20-A21. The new peptide was obtained by substitution of two aromatic L-amino acid residues (B24 and B26) for their D-optical isomers. These peptides were tested with cell cultures L929 and PC12 (glucose uptake). Increased concentration of peptides correlated with stimulation of glucose uptake by cells. Studies carried out on animals with streptosotocine-caused diabetes showed that, synthesized peptides were able to decrease glucose level in blood, but decapeptide with D amino acid showed a more pronounced effect compared to the decapeptide with L amino acid.
Download PDF:
Citation:
Maslov D.L., Lokhov P.G., Abakumova O. YU., Tsvetkova T.A., Prozorovskiy V.N. (2002) The prolonged action of insulin analogue by the substitution of L-amino acids fortheir D-isomers. Voprosy Meditsinskoi Khimii, 48(1), 103-110.
Maslov D.L. et al. The prolonged action of insulin analogue by the substitution of L-amino acids fortheir D-isomers // Voprosy Meditsinskoi Khimii. - 2002. - V. 48. -N 1. - P. 103-110.
Maslov D.L. et al., "The prolonged action of insulin analogue by the substitution of L-amino acids fortheir D-isomers." Voprosy Meditsinskoi Khimii 48.1 (2002): 103-110.
Maslov, D. L., Lokhov, P. G., Abakumova, O. , YU., Tsvetkova, T. A., Prozorovskiy, V. N. (2002). The prolonged action of insulin analogue by the substitution of L-amino acids fortheir D-isomers. Voprosy Meditsinskoi Khimii, 48(1), 103-110.
References
Nasri F., Dib S.A., Sa J.R. et al. (1995) Rev Assoc. Med Bras, 41, 37- 42. Scholar google search
Wichterle D., Stolba P., Bendlova B. (1995) Vnitr Lek.,41,146- 150. Scholar google search
Harrpop M., Caudwell J., Colman P.G. (1992) Diabetes Res Clin. Pract., 18, 107- 112. Scholar google search
Hao W., Serreze D.V., Palmer J.P. (1993) J. Autoimmun., 6,787- 798. Scholar google search
Crowther N.J., Xiao В., Hales C.N. (1994) Protein Engineering,, 7,137- 144. Scholar google search
Kurtzhals P., Kiehr B., Sorensen A. (1995) J. Pharm. Sci., 84,1164- 1168. Scholar google search
Kobayas M.M., Ohgaku S., Iwasaki M., Maegawa H. (1983) Current And Future Therapies With Insulin (Sokamoto M., Alberti K.G., Eds.), pp. 136- 141, Excerpta Med., Amsterdam. Scholar google search
Devenyi Т., Gergefy J. (1974) Amino Acids, Peptides and Proteins, p. 235, Elsevier Scientific Publishing Company, Amsterdam- London- New ^fork. Scholar google search
Cooper J.D., Ogden G., McIntosh and Turnell D.C. (1984) Analyt. Biochem., 142,98- 102. Scholar google search
