VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Monoclonal anti-NSE-antibodies: purification,characterization and immunoenzyme analisys

   
Chekhonin V.P.1, Gurina O.I.1, Portnaja T.S.1, Rjabukhin I.A.1, Shepeleva I.I.1

1. Serbsky National Research Centre for Social and Forensic Psychiatry
PubMed Id: 12498090
Year: 2002  Volume: 48  Issue: 5  Pages: 477-484
The results of NSE purificationprocedure, as well as hybridoma technology of anti NSE monoclonal antibodies synthesis arepresented. The employment of this procedure yielded highly purified NSE preparation. Theimmunization of BALB/C mice with NSE preparation led to sensitization of theimmunocompetent cells, which could form hybridomes, producing the anti-NSE monoclonalantibodies, after the confluence with myeloma cells Sp 2/0-Ag 14.
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Chekhonin V.P., Gurina O.I., Portnaja T.S., Rjabukhin I.A., Shepeleva I.I. (2002) Voprosy meditsinskoi khimii, 48(5), 477-484.
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