A procedure of isolation of human blood plasma prekallikrein, coagulation factor XII and active fragment beta-XIIa has been developed. This procedure includes the traditional chromatography steps and FPLC. Disc-electrophoresis revealed that the preparations of factor XII and beta-XIIa were homogeneous. Their specific activity was 70.6 U and 2.5 U, respectively. The procedure described is less time consuming and it allows to isolate these factors in the preparative quantities.
Download PDF:
Citation:
Blokhina T.B., Neshkova E.A., Yarovaya G.A. (2002) Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa). Voprosy Meditsinskoi Khimii, 48(6), 603-610.
Blokhina T.B. et al. Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa) // Voprosy Meditsinskoi Khimii. - 2002. - V. 48. -N 6. - P. 603-610.
Blokhina T.B. et al., "Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa)." Voprosy Meditsinskoi Khimii 48.6 (2002): 603-610.
Blokhina, T. B., Neshkova, E. A., Yarovaya, G. A. (2002). Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa). Voprosy Meditsinskoi Khimii, 48(6), 603-610.
Weiss A., Galiin J., Kaplan A. (1974) J. Clin. Invest, 53, 622- 633. Scholar google search
Dotsenko V.L., Nenasheva N.M., Neshkova E.A., Yarovaya G.A. (1989) Advances in Experimental Medicine and Biology, Kinin V Ed. Abe K., Moriya H., Fumii S., New York: Plenum Press, B, 515- 521. Scholar google search
Silverberg M., Kaplan A.P. (1988) Metods in enzymology, 163M, 68- 78. Scholar google search
Kaplan A.P, Meyer M.L., Mandle R.J. (1977) Mediators of the Immediate Type Inflairitatory Reaction /Monographs in Allergy/, 120- 131. Scholar google search
Schiffman S., Rapaport S.T., Uare A.G. (I960) Proc. Soc. Exp. Biol. Med., 105, 453- 465. Scholar google search
Ratnoff O.D., Davie E.W. (1961) J. Clin. Unvest, 41, 803- 824. Scholar google search
