Characterization of endogenous cysteineproteinase inhibitors from transformed fibroblasts

Dilakian E.A.1, Gureeva T.A.1, Solovyeva N.I.1, Zhurbitskaya V.A.2, Suletskaya Yu.A.1

1. Institute of Biomedical Chemistry Russian Academy of Medical Sciences (RAMS)
2. Institute of Carcinogenesis, Russian Cancer Research Center, RAMS
PubMed Id: 12698562
Year: 2002  Volume: 48  Issue: 6  Pages: 611-617
The thermostable endogenous cysteine proteinase inhibitors (CPI) from the primary (REF), immortal (clone IE5) and transformed (clones trF8 and trF8nmcc) fibroblasts were isolated. All the isolated CPI act as reversible competitive inhibitors of cathepsins B and L and of papain. The study of inhibition of cathepsins B and L, purified from the same cell cultures as the CPI, showed that the Ki values for CPI from the cultures of immortal and transformed cells were by one order higher than the Ki values for CPI of primary fibroblasts. The data obtained suggest that immortalization and transformation alter the CPI properties.
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Dilakian, E. A., Gureeva, T. A., Solovyeva, N. I., Zhurbitskaya, V. A., Suletskaya, Yu. A. (2002). Characterization of endogenous cysteineproteinase inhibitors from transformed fibroblasts. Voprosy Meditsinskoi Khimii, 48(6), 611-617.
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