VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Determination of functional activity of components and factors of the human complement

   
Kozlov L.V.1

1. G.N.Gabrichevsky Moscow scientific research institute of epidemiology and microbiology
PubMed Id: 12698564
Year: 2002  Volume: 48  Issue: 6  Pages: 624-631
Development suitable for clinical researches of hemolytic methods of determination of functional activity of the first components of a complement has allowed to show diagnostic value of testing activity of complement components in comparison with their contents as antigens. It has predetermined necessity for building modern ELISA tests-systems for quantitative determination of functional activity of complement components. Such methods built for the first time allow to determine activity of components C1q, C2, C3, C4 (and a ratio of isotypes C4A and C4B), C1 - inhibitor, factors B and D. Addition of these tests-systems ELISA systems for quantitative determination of components, and in case of С1-inhibitor of presence IgG, IgA and IgM autoantibodies against С1-inhibitor frames opportunities of an evaluation complement status of the patient, hereditary predisposition to such diseases as a stomach ulcer, the glaucoma, a clamidiosis, bacteroidosis, allows to carry out differential diagnostics of angioedema. Inhibition of covalent linkage C4b or C3b various endogenic and exogenous effectors during formation С3-and С5-convertases allows to understand processes of a regulation of a homeostasis, and also the mechanism of action of drugs
Download PDF:
Reference:

Kozlov L.V. (2002) Voprosy meditsinskoi khimii, 48(6), 624-631.
References  
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)
        

© Institute of Biomedical Chemistry, Moscow, Russia