A cell population, characterized by a capacity to survive in the absence of cell attachment to a substrate (anoikis-independent cells), was selected from the original anoikis-dependent human gut carcinoma line Caco-2. In cell-adhesion assays, anoikis-independent cells demonstrated much lower affinity to fibronectin compared to their anoikis-dependent counterparts. No differences were found between the cell types in their surface expression of integrins alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, and alpha 6 beta 1, while integrins alpha v beta 1 and alpha v beta 3 appeared to be much more actively expressed by the anoikis-dependent population. The procedure we have proposed for obtaining of cell populations having closely similar origination, while differing in anchorage-dependent apoptosis, provides a suitable model for investigation of apoptotis and the role of integrins in its mechanism.
Morozevich G.E., Kozlova N.I., Berman A.E. (1998) Expression of integrins in human gut carcinoma cells, differing in anchorage dependent apoptosis. Voprosy Meditsinskoi Khimii, 44(1), 77-83.
Morozevich G.E. et al. Expression of integrins in human gut carcinoma cells, differing in anchorage dependent apoptosis // Voprosy Meditsinskoi Khimii. - 1998. - V. 44. -N 1. - P. 77-83.
Morozevich G.E. et al., "Expression of integrins in human gut carcinoma cells, differing in anchorage dependent apoptosis." Voprosy Meditsinskoi Khimii 44.1 (1998): 77-83.
Morozevich, G. E., Kozlova, N. I., Berman, A. E. (1998). Expression of integrins in human gut carcinoma cells, differing in anchorage dependent apoptosis. Voprosy Meditsinskoi Khimii, 44(1), 77-83.
