VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Expression of integrins in human gut carcinoma cells, differing in anchorage dependent apoptosis

   
Morozevich G.E., Kozlova N.I., Berman A.E.
PubMed Id: 9575616
Year: 1998  Volume: 44  Issue: 1  Pages: 77-83
A cell population, characterized by a capacity to survive in the absence of cell attachment to a substrate (anoikis-independent cells), was selected from the original anoikis-dependent human gut carcinoma line Caco-2. In cell-adhesion assays, anoikis-independent cells demonstrated much lower affinity to fibronectin compared to their anoikis-dependent counterparts. No differences were found between the cell types in their surface expression of integrins alpha 1 beta 1, alpha 2 beta 1, alpha 3 beta 1, alpha 5 beta 1, and alpha 6 beta 1, while integrins alpha v beta 1 and alpha v beta 3 appeared to be much more actively expressed by the anoikis-dependent population. The procedure we have proposed for obtaining of cell populations having closely similar origination, while differing in anchorage-dependent apoptosis, provides a suitable model for investigation of apoptotis and the role of integrins in its mechanism.
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Keywords: integrins, apoptosis, substrate dependence, anoikis, cell adhesion
Citation:

Morozevich, G. E., Kozlova, N. I., Berman, A. E. (1998). Expression of integrins in human gut carcinoma cells, differing in anchorage dependent apoptosis. Voprosy meditsinskoi khimii, 44(1), 77-83.
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