VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Isolation and some properties of rat skeletal muscle polysomes

   
Shishkin S.S., Kitavtsev B.A., Debov S.S.
PubMed Id: 1138499
Year: 1975  Volume: 21  Issue: 2  Pages: 137-142
Possibility was investigated to prepare polysomes from microsomal fraction of rat sceletal muscles. Centrifugation in sucrose gradient showed that approximately 60 percent of UV-absorbing material in the preparation obtained was in polysomic region of the gradient. Mild treatment with pancreatic ribonuclease led to disintegration of polysomes and respective increase in content of monosomic component. The use of combined gradient permitted to obtain two fractions of polysomes, one of which was readily sedimented by centrifugation. This fraction contained significant amount of protein and incorporated relatively low quantity of 32-P within 2 hrs after administration of the label in vivo. The data obtained suggest that the fraction of polysomes carried out the biosynthesis of myosin.
Download PDF:
Reference:

Shishkin S.S., Kitavtsev B.A., Debov S.S. (1975) Voprosy meditsinskoi khimii, 21(2), 137-142.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)
        

© Institute of Biomedical Chemistry, Moscow, Russia