VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Analysis of inhibition in pathways of NADP.H2 and NAD.H2 oxidation in liver tissue microsomes

   
Archakov A.I., Ahirnov G.E., Karuzina I.I.
PubMed Id: 1902
Year: 1975  Volume: 21  Issue: 3  Pages: 281-285
Microsomal complexes of electron transfer were resistant to typical inhibitors of mitochondrial pathway of electron transport. In oxidation of NADP.H2 there were at least three point of molecular O2 reduction: NADP.H2-specific flavoprotein, Fe2+ participating in reactions of peroxidation of unsaturated fatty acids and cytochrome P-450. Efficiency of cytochrome P-450 inhibitors could not be evaluated by polarography as in the pathway several sites of molecular O2 activation were observed. In oxidation of NADP.H2 estimation of the rate of electron transfer reactions was carried out by monitoring of velocity of O2 absorption in presence of EDTA (inhibitor of the reaction of peroxidation) because about 50% of the total oxygen were utilized only in the process where NADP.H2 was oxidized. NAD.H2 oxidation, inhibited with EDTA, was activated by addition of Ca2+.
Download PDF:  
Citation:

Archakov, A. I., Ahirnov, G. E., Karuzina, I. I. (1975). Analysis of inhibition in pathways of NADP.H2 and NAD.H2 oxidation in liver tissue microsomes. Voprosy Meditsinskoi Khimii, 21(3), 281-285.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)
◄        ►