VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Simultaneous estimation of catecholamines and serotonin after purification on ion exchange resin

   
Manukhin B.N., Berdysheva L.V., Volina E.V.
PubMed Id: 1210109
Year: 1975  Volume: 21  Issue: 3  Pages: 317-321
A general fluorimetric method was developed for determination of noradrenaline, adrenaline, dopamine and serotonin in a single sample. Purification of samples was carried out on columns of Dowex 50X4 and 10 in sodium form. As judget by an internal standard the yield of noradrenaline and adrenaline was about 90% and of dopamin -- about 75%. Serotonin was determined by means of a condensation reaction with ninhydrin. A more stable fluorescence of serotonin samples was provided by an increase in warming-up time up to 55 min. Treatment of control probes with iodine solution permitted to eliminate an unspecific fluoresence of the tissues studied. The yield of serotonin was about 75% as estimated by an internal standard.
Download PDF:
Citation:

Manukhin, B. N., Berdysheva, L. V., Volina, E. V. (1975). Simultaneous estimation of catecholamines and serotonin after purification on ion exchange resin. Voprosy meditsinskoi khimii, 21(3), 317-321.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)