VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Express microanalysis of total lipids and their fractions in blood serum by micro-thin layer chromatography

   
Gribanov G.A., Sergeev S.A.
PubMed Id: 1216781
Year: 1975  Volume: 21  Issue: 6  Pages: 652-655
A rapid and sensitive microanalysis in blood serum of total lipids and their main fractions (phospholipids, free cholesterol, free fatty acids, triglycerids, esters of cholesterol) by means of micro-thin layer chromatography is described. The quantitative determination of total lipids and their fractions, after elution from the chromatogramms, was carried out by means of degradation with concentrated sulphuric acid and subsequent photometric estimation at 400 nm. The method enabled to determine from 15-20 mcg to 180-200 mcg of total lipids and their fractions. The error of the method did not exceed +/-8.5% for total lipids.
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Reference:

Gribanov G.A., Sergeev S.A. (1975) Voprosy meditsinskoi khimii, 21(6), 652-655.
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