VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Isoelectric focusing in a boroborate buffer-mannitol system (BBM)

   
Troitskiĭ G.V., Azhitskiĭ G.Yu., Malyi D.D.
PubMed Id: 16397
Year: 1976  Volume: 22  Issue: 2  Pages: 282-286
The data of the following study of isoelectric focusing in the system 'buffer--nonelectrolyte' are described. The method was shown to be employed for study of proteins, when human serum albumin was used as a model. A standard method and an apparatus device are proposed for the analytical isoelectric focusing of proteins both in the wide (pH 4.00-8.50) and in the limit (PH 4.50-6.00) PH regions. Advantages and limitations of the method are discussed as compared with the method of isoelectric focusing in ampholines.
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Troitskiĭ, G. V., Azhitskiĭ, G. Yu., Malyi, D. D. (1976). Isoelectric focusing in a boroborate buffer-mannitol system (BBM). Voprosy Meditsinskoi Khimii, 22(2), 282-286.
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