VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Enzyme splitting S-adenosylmethionine in the cells of Escherichia coli CK infected with phage CD

   
Nikol'skaia I.I., Debov S.S.
PubMed Id: 799869
Year: 1976  Volume: 22  Issue: 3  Pages: 343-346
Extract of normal Escherichia coli CK cells methylated DNA of Cd phage in vitro in presence of 3H-S-adenosyl methionine, although in vivo methylation of viral DNA did not occur. Extract of cells, infected with Cd phage, also was unable to methylate the DNA in vitro due to de novo synthesis of the enzyme splitting S-adenosyl methionine to 5'-methyl thioadenosine and homoserine. The enzyme was not found in cells, infected with the phage in presence of chloramphenicol. The activity of the S-adenosyl methionine splitting enzyme was shown to be maximal between the fourth and fifth min of infection. This suggests that the enzyme is synthesized under control of the virus genome, it belongs to the early virus-specific proteins and inhibits the methylation of the phage DNA in vivo. In unpurified extracts of Escherichia coli CK the methylating activity was completely inhibited by 10(-5) M S-adenosyl homocysteine.
Download PDF:  
Citation:

Nikol'skaia, I. I., Debov, S. S. (1976). Enzyme splitting S-adenosylmethionine in the cells of Escherichia coli CK infected with phage CD. Voprosy Meditsinskoi Khimii, 22(3), 343-346.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)
◄        ►