VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Comparative study of 21S RNP particles and ribosomes from the cytoplasm of the cells of sarcoma M-1 in vitro

   
Roslyĭ I.M., Votrin I.I.
PubMed Id: 1030894
Year: 1976  Volume: 22  Issue: 3  Pages: 366-374
Preparations of RNP-particles from rat sarcoma M-1 were obtained by means of treatment with different detergents: anione - sodium deoxycholate (1%) and non-ionic - Triton X-100 (1%). Triton X-100 caused some increase in content of the small ribosomal subunit and also in the content of 21 S and 7.5 S particles in the preparation. The latter particles as well as ribosomes were resistant to different detergents. 21 S RNP-particles were stable in 1 M KCl. In a cell-free system 21 S and 7.5 S RNP-particles lost a significant amount of labelled protein, which appeared partially in the ribosomes.
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Roslyĭ, I. M., Votrin, I. I. (1976). Comparative study of 21S RNP particles and ribosomes from the cytoplasm of the cells of sarcoma M-1 in vitro. Voprosy Meditsinskoi Khimii, 22(3), 366-374.
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