Use of proteins, covalently bound to agar gel, for specific sorption in electrophoresis

Kuzovleva O.B., Nikolaeva A.I.
PubMed Id: 67710
Year: 1976  Volume: 22  Issue: 3  Pages: 413-417
A method is developed for electrophoretic fractionation of serum proteins in agar gel with simultaneous specific sorption of gamma-globulin fraction, migrating during the electrophoresis through specific immunosorbents incorporated into agar gel. The reaction was carried out in a special apparatus designed for the electrophoresis in two perpendicular directions. The sorbent was separated from impurities by passing the current in the first direction. The unspecific sorbent did not alter the electrophoretic mobility of migrating proteins, but the specific sorbents immobilized completely both antibodies and antigens. The antibodies were shown to bind repeatedly to the immunoglobulins, which had been linked to the sorbent. The method facilitated distinctly the estimation of antigens and antibodies in complicated mixtures. By analogy with affinity chromatography a term 'affinity electrophoresis' was used for designation of the method described.
Download PDF:

Kuzovleva, O. B., Nikolaeva, A. I. (1976). Use of proteins, covalently bound to agar gel, for specific sorption in electrophoresis. Voprosy meditsinskoi khimii, 22(3), 413-417.
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)