VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Chemical composition of casein glycomacropeptide components

   
Varsanovich E.A., Usacheva N.T., Stan E.Ia., Vasil'ev A.V., Chernikov M.P.
PubMed Id: 1014475
Year: 1976  Volume: 22  Issue: 5  Pages: 648-652
Alteration in pH and addition of urea (final concentration 3 M) did not affect the electrophoretic and chromatographic separation of glycomacropeptide. At the same time, urea (5 M) distinctly increased an amount of beta-component in separation by free electrophoresis. Sedimentation constant SO2OW and molecular weight of glycomacropeptide were equal to 1.35 S and 13000, respectively. Both fractions of glycomacropeptide had similar amino acid composition and contained phosphorus (0.4%). The variations were observed in the carbohydrate components of the molecules. The heavy component contained 5-fold higher concentration of sialic acids (9.7-9.6%), 2-fold higher concentration of galactose (13%) and acetyl galactosamine (5.0-4.0%) as compared with the beta-component (3.1-0.9%, 7.5% and 2.5%, respectively). These alterations in carbohydrate component of glycomacropeptide were suggested to affect the polymeric conformation of the molecule.
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Varsanovich E.A., Usacheva N.T., Stan E.Ia., Vasil'ev A.V., Chernikov M.P. (1976) Voprosy meditsinskoi khimii, 22(5), 648-652.
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