VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Native and artificial sub-forms of cytoplasmic aspartate transaminase from swine heart

   
Denisova G.F., Polianovskiĭ O.L.
PubMed Id: 1014476
Year: 1976  Volume: 22  Issue: 5  Pages: 652-655
Multiple forms of aspartate transaminase from cytosol of pig heart (alpha, beta and psi) were incubated in 0.05 M acetate buffer, pH 5.0 at 4 degrees within 5 and 8 months. Gradual accumulation of denaturated forms was observed on incubation of alpha- and beta-forms; these forms possessed higher mobility in polyacrylamide gel disc electrophoresis and decreased enzymatic activity as compared with the native forms. Multiple forms, produced during ageing of alpha-form, were separated by chromatography on CM-cellulose. Chromatographic resolution of the forms, their activity and spectral properties suggest that they are not identical with the native isoenzymes found in the cell.
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Denisova, G. F., Polianovskiĭ, O. L. (1976). Native and artificial sub-forms of cytoplasmic aspartate transaminase from swine heart. Voprosy Meditsinskoi Khimii, 22(5), 652-655.
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