Sequence of conversion of pregnenolone into androgens in virilizing adrenal glands in man

Iudaev N.A., Smirnova N.B.
PubMed Id: 189503
Year: 1976  Volume: 22  Issue: 5  Pages: 673-678
Trace amounts of labelled pregnenolone were added to the slices of veriliscent tumor from human adrenal gland in order to study the stepwise conversion of the steroid into androstenedione, 11betaOH-androstenedione and their intermediats--17-alpha-OH pregnenolone, dehydroepiandrosterone, progesterone, and 17-alpha-OH-progesterone. The definite sequence was observed in the maximal incorporation of pregnenolone label into 17-alpha-OH-pregnenolone (2-5 min of incubation) leads to dehydroepiandrosterone (5-15 min) leads to androstenedione (10-20 min). Incorporation of the pregnenolone label into 17-alpha-OH-pregnenolone and dehydroepiandrosterone was distinctly higher than into progesterone and 17-alpha-OH-progesterone in all the experiments within all the studied periods of incubation. The data obtained suggest that in viriliscent tumors of adrenal gland conversion of pregnenolone into androgens proceeded as follows: pregnenolone leads to 17-alpha-OH-pregnenolone leads to dehydroepiandrosterone leads to androstenedione. When formation of androstenedione and 11beta-OH-androstenedione from pregnenolone and progesterone was analyzed during the experiment it was shown that 11-beta-OH-androstenedione was mainly formed via androstenedione and the hormone was the end product of the androgen synthesis.
Download PDF:  

Iudaev, N. A., Smirnova, N. B. (1976). Sequence of conversion of pregnenolone into androgens in virilizing adrenal glands in man. Voprosy Meditsinskoi Khimii, 22(5), 673-678.
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)