VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Fractionation of DNA on unmodified cellulose

   
Kotin A.M., Teriukova N.P.
PubMed Id: 1014486
Year: 1976  Volume: 22  Issue: 5  Pages: 712-716
Not only separation of native, two-stranded DNA from denatured molecules but more distinct fractionation of single-stranded DNA was demonstrated on unmodified powdery and microgranular cellulose. Denatured DNA, absorbed on cellulose, was eluted from a column as two asymmetric peaks by 0,12 M and 0.05 M NaCl (for I and II peaks, respectively). Absorption and fractionation of denatured DNA and poly-A containing RNA on unmodified cellulose was apparently achieved on the basis of the same principles.
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Kotin, A. M., Teriukova, N. P. (1976). Fractionation of DNA on unmodified cellulose. Voprosy Meditsinskoi Khimii, 22(5), 712-716.
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