VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Native and desensitized forms of L-type pyruvate kinase from rabbit kidney cortex

   
Kozhevnikova K.A.
PubMed Id: 595502
Year: 1977  Volume: 23  Issue: 5  Pages: 658-661
A time-consuming procedure of isolation of pyruvate kinase isoenzymes from rabbit kidney cortex (more than 5 hrs) at 0-2 degrees led to obtaining of a desensitized form of 'L' type, resembling the 'M3' type of the enzyme from sceletal muscle. Rapid isolation of pyruvate kinase 'L' type (within about 2.5 hrs) at 4-6 degrees provided the isoenzyme in the active (allosteric) form.
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Kozhevnikova, K. A. (1977). Native and desensitized forms of L-type pyruvate kinase from rabbit kidney cortex. Voprosy Meditsinskoi Khimii, 23(5), 658-661.
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