VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Quantitative determination of tissue disulfide groups by reverse amperometric titration

   
Sokolovsky V.V., Belozerova L.A., Ogurtsova R.E.
PubMed Id: 595506
Year: 1977  Volume: 23  Issue: 5  Pages: 709-712
A method of reversed amperometric titration for estimation of content of S- S-groups in biological materials is described. The method is based on splitting of disulphide groups in reaction with a reducing agent (Na2SO3) in presence of an excess of metal ions (Ag+, Hg2+), which form a mercaptide bond (AgS) with SH-groups. The excess of free metal ions was titrated with the equimolar solution of thiol (unithiol, cysteine). The method is comparatively simple and enables to estimate S- S groups in presence of air oxygen and enzymatic systems.
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Sokolovsky, V. V., Belozerova, L. A., Ogurtsova, R. E. (1977). Quantitative determination of tissue disulfide groups by reverse amperometric titration. Voprosy Meditsinskoi Khimii, 23(5), 709-712.
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