VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Adenylate kinase and creatine kinase activity of rabbit blood serum following application of a tourniquet to the thigh

   
Chetverikova E.P.
PubMed Id: 202085
Year: 1977  Volume: 23  Issue: 6  Pages: 728-732
A colorimetric method is described for estimation of the adenilate kinase activity in blood serum; the method is based on coupling of adenilate- and creatine kinase reactions and on estimation of the amount of creatine formed. Adenilate kinase activity in blood serum, estimated by the method, was shown to increase 4-fold after removing of tourniquet with subsequent normalization within the next day. The same data were obtained using a spectrophotometric method for estimation of adenilate kinase based on NADP reduction in coupled reactions with hexokinase and glucose-6-phosphate dehydrogenase. An increase in creatine kinase activity in blood serum occurred later on after removing of the tourniquet; it was more distinct (8.5-fold) and maintained longer as compared with the increase in adenilate kinase activity.
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Chetverikova, E. P. (1977). Adenylate kinase and creatine kinase activity of rabbit blood serum following application of a tourniquet to the thigh. Voprosy Meditsinskoi Khimii, 23(6), 728-732.
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