Specific radioactivity of RNA from rat liver tissue, labelled with 14C-orotic acid and fractionated using thermic phenol method, was altered dissimilarly. In single stimulation of hypothalamus the specific radioactivity of nuclear RNA was increased in RNA-40 degrees fraction than in RNA-55 degrees and RNA-63 degrees ones. A decrease in synthesis of nuclear RNA fractions followed the phase of activation in repeated stimulation of hypothalamus. The distinct increase in specific radioactivity was observed in cytoplasmic RNA (RNA-4 degrees) both in single and repeated stimulation of hypothalamus. The stimulating effect of the prolonged hypothalamus irritation on synthesis of the RNA fractions and on activity of glucose-6-phosphatase and fructose-1,6-diphosphatase disappeared more rapidly than this effect in long-term administration of hydrocortisone. The data obtained suggest that sensitivity of tissue-targets to prolonged hormonal stimulation is distinctly higher than the ability of hypothalamus to activate the synthesis of RNA and enzymatic proteins.
Frol'kis V.V. et al. Synthesis of RNA fractions in rat liver during single and multiple stimulation of the hypothalamus // Voprosy meditsinskoi khimii. - 1978. - V. 24. -N 1. - P. 12-17.
Frol'kis V.V. et al., "Synthesis of RNA fractions in rat liver during single and multiple stimulation of the hypothalamus." Voprosy meditsinskoi khimii 24.1 (1978): 12-17.
Frol'kis, V. V., Bezrukov, V. V., Muradian, Kh. K. (1978). Synthesis of RNA fractions in rat liver during single and multiple stimulation of the hypothalamus. Voprosy meditsinskoi khimii, 24(1), 12-17.