Biosynthesis of pyridoxal-5'-phosphate in lymphocytes stimulated by alloantigens in vitro

Sergeev A.V., Bykovskaia S.N., Raushenbakh M.O.
PubMed Id: 307304
Year: 1978  Volume: 24  Issue: 2  Pages: 211-215
Dynamics of alterations in pyridoxal kinase activity (key enzyme in biosynthesis of pyridoxal-5'-phosphate) was studied in lymphocytes of mice BALB/c, stimulated by irradiated cells from spleen of mice C3H in vitro. The cytolytic activity of lymphocytes was estimated by amount of Na251CrO4 realised from lysed cell-targets into medium. Maintenance of animals on a diet without pyridoxine within 1-3 weeks did not affect on the capacity of lymphocytes to proliferative in vitro and on their cytolytic activity. At the same time pyridoxal kinase activity was increased 2.5-3-fold in stimulated lymphocytes in presence of pyridoxal. Km value was 2.5-5.0.10(-5) M of pyridoxal for preparations of pyridoxal kinase from lymphocytes either with normal or with increased enzymatic activity. Lymphocytes of mice, kept on the diet without pyridoxine within 5-6 weeks, were characterized by distinctly decreased proliferative and cytolytic activities. Pyridoxal-5'-phosphate, added to incubation mixture in vitro, as distinct from pyridoxal, restored partially the impaired functions of T-lymphocytes.
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Sergeev, A. V., Bykovskaia, S. N., Raushenbakh, M. O. (1978). Biosynthesis of pyridoxal-5'-phosphate in lymphocytes stimulated by alloantigens in vitro. Voprosy Meditsinskoi Khimii, 24(2), 211-215.
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