VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Determination of hexokinase isoenzymes in different tissues

   
Shurda G.G., Panin L.E.
PubMed Id: 758095
Year: 1978  Volume: 24  Issue: 2  Pages: 282-285
A modified method is described for estimation of hexokinase isoenzymes by polyacrylamide gel electrophoresis. The best conditions for detection of hexokinase activity were developed using immobilization of glucose-6-phosphate dehydrogenas: in gel, utilization of riboflavin as a catalyst of polymerization and stabilization by glucose. The method is simple, highly reproducible, comparatively rapid and economically reasonable.
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Shurda, G. G., Panin, L. E. (1978). Determination of hexokinase isoenzymes in different tissues. Voprosy Meditsinskoi Khimii, 24(2), 282-285.
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