VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Study of the toxic factor in liver extracts

   
Gabrielian N.I., Mogireva I.A.
PubMed Id: 664463
Year: 1978  Volume: 24  Issue: 3  Pages: 341-345
Dissolution of mitochondrial fraction of ischemic liver was carried out using various detergents, the most effective of which was sodium dodecylsulphate (SDS). Excess of SDS was removed by dialysis or by sorption on anion exchange resin. The optically homogenous solution obtained was subsequently purified by gel filtration on Sephadex G-75. The preparation contained two fractions: the molecular weight of the major fraction corresponded to that of gamma-globulin and the molecular weight of the minor fraction--to that of cytochrome C. Interrelation was observed between the toxic activity and hydrophobic components of the fraction isolated.
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Gabrielian, N. I., Mogireva, I. A. (1978). Study of the toxic factor in liver extracts. Voprosy Meditsinskoi Khimii, 24(3), 341-345.
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