VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Effect of low temperatures on the functional state of the membranes of the hepatic endoplasmic reticulum

   
Makevnina M.G., Kudokotseva E.V., Dobretsov G.E., Belous A.M.
PubMed Id: 30211
Year: 1978  Volume: 24  Issue: 5  Pages: 605-608
Oxidation of fluorescent substrates--NADPH and 4-dimethylamine chalcone (DMC) by microsomes from liver endoplasmic reticulum was studied after freezing and thawing of these cells organelles. Slow freezing to -25 degrees or quick freezing to -196 degrees did not affect distinctly the rate of NADPH oxidation after thawing, but oxidation of DMC by microsomes was decreased in both cases. Freezing and subsequent thawing of microsomes impaired apparently the terminal sites of electron transfer and/or the systems of hydroxylation, including cytochrome P-450. Slow freezing to -25 degrees impaired microsomes more markedly than quick freezing to -196 degrees.
Download PDF:  
Citation:

Makevnina, M. G., Kudokotseva, E. V., Dobretsov, G. E., Belous, A. M. (1978). Effect of low temperatures on the functional state of the membranes of the hepatic endoplasmic reticulum. Voprosy Meditsinskoi Khimii, 24(5), 605-608.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)
◄        ►