VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Use of alpha-ketobutyrate for the differential determination of the activities of lactate dehydrogenase isoenzyme subunits

   
Koniukhova L.K., Malakhov V.N.
PubMed Id: 452487
Year: 1979  Volume: 25  Issue: 3  Pages: 245-250
Estimation of alpha-ketobutyrate dehydrogenase activity might be used for quantitative evaluation of LDH isozyme subunits H and M. Empiric constants, which are included in equations for calculation of the activity of subunits and applied for purified human LDH isoenzymes, were not altered in presence of blood serum and were quite reproducible from day to day. Reliability of quantitative differentiation of the activities of H and M subunits by means of alpha-ketobutyrate was demonstrated by analyses of standard LDH isoenzyme mixtures as well as normal and pathological blood sera with typical patterns of LDH isoenzymes. Estimation of activity of the LDH isoenzyme subunits using alpha-ketobutyrat e proved to be rapid, simple and unexpensive method for determination of LDH isoenzyme spectra in physiological fluids
Download PDF:  
Citation:

Koniukhova, L. K., Malakhov, V. N. (1979). Use of alpha-ketobutyrate for the differential determination of the activities of lactate dehydrogenase isoenzyme subunits. Voprosy Meditsinskoi Khimii, 25(3), 245-250.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)
◄        ►