VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Determination of somatotropin and prolactin by dodecyl sulfate polyacrylamide gel electrophoresis

   
Shostak I.N., Rozhko O.T., Martynenko F.P.
PubMed Id: 7368631
Year: 1980  Volume: 26  Issue: 1  Pages: 71-75
Definite separation of somatotropin and prolactin using sodium dodecylsulphate polyacrylamide gel electrophoresis takes place in continuous systems with pH 7.0-7.2 containing acetate or chloride monovalet anions; substitution of the ions by polyvalent phosphate or borate anions interfered with the separation. The hormones precipitated by trichloracetic acid may be separated and quantitatively determined after dissolving in the weak alkaline solutions. The hormones are distinctly separated from serum proteins and may be quantitatively determined in media containing up to 15% of serum.
Download PDF:  
Citation:

Shostak, I. N., Rozhko, O. T., Martynenko, F. P. (1980). Determination of somatotropin and prolactin by dodecyl sulfate polyacrylamide gel electrophoresis. Voprosy Meditsinskoi Khimii, 26(1), 71-75.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)
◄        ►