VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Simultaneous extraction and fluorimetric determination of noradrenaline, dopamine, serotonin and 5-hydroxyindoleacetic acid in separate small brain segments

   
Kocherga V.I., Opentanova I.L.
PubMed Id: 6154385
Year: 1980  Volume: 26  Issue: 1  Pages: 81-85
Highly-sensitive and reproducible method is developed for simultaneous assay of noradrenaline, dopamine, serotonin and 5-hydroxyindolylacetic acid in small samples of brain tissue. The procedure comprised extraction of the substances with acidified butanol, reextraction of the compounds with phosphate buffer in presence of isooctane, separation of the amines form precursors and metabolites using ion exchange resin Amberlite CG-50 and of 5-hydroxyindolylacetic acid--on Sephadex G-10.
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Kocherga, V. I., Opentanova, I. L. (1980). Simultaneous extraction and fluorimetric determination of noradrenaline, dopamine, serotonin and 5-hydroxyindoleacetic acid in separate small brain segments. Voprosy Meditsinskoi Khimii, 26(1), 81-85.
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