VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Isolation and radiometric determination of rat liver ATP: thiamine diphosphate phosphotransferase activity

   
Voskoboev A.I., Luchko V.S.
PubMed Id: 6256962
Year: 1980  Volume: 26  Issue: 4  Pages: 564-568
A radioassay was developed for estimation of the thiamin diphosphate kinase activity (EC 2.7.4.15); 14C-TDP was used as a substrate. After termination of the reaction (formation of TTP), the products obtained were separated using ion exchange chromatography on SP-Sephadex C-25. The method developed was very sensitive and enabled to estimate the enzymatic activity in tissue homogenates. TDP-kinase was isolated from rat liver with a 70-fold purification. Dependence of the reaction rate on pH value, concentrations of the enzyme and thiamin were studied.
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Voskoboev, A. I., Luchko, V. S. (1980). Isolation and radiometric determination of rat liver ATP: thiamine diphosphate phosphotransferase activity. Voprosy Meditsinskoi Khimii, 26(4), 564-568.
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