VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Elimination of cholesterol by affinity chromatography

   
Berezin I.V., Lopukhin I.M., Andrianov I.P., Lapuk I.I., Alekseeva L.B.
PubMed Id: 7456416
Year: 1980  Volume: 26  Issue: 6  Pages: 843-846
Elimination of cholesterol from physiological fluids was carried out by means of affinity chromatography using digitonine as an affinity agent. For this purpose a method of covalent binding of digitonine with aminosylochrome was developed. The most suitable conditions for production of the stable sorbent specific for cholesterol are described. The ratio of concentrations of digitonine to sodium periodate was 1:2; oxidation was performed in a medium containing 50% aqueous dioxane within 1.5 hrs. The modified sorbent, used for affinity chromatography of cholesterol, possessed a specific capacity of 10 mg/g.
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Berezin I.V., Lopukhin I.M., Andrianov I.P., Lapuk I.I., Alekseeva L.B. (1980) Voprosy meditsinskoi khimii, 26(6), 843-846.
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