VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Isolation of the theta-hemolysin of Cl. perfringens

   
Dolgikh M.S., Blagoveshchenskiĭ V.A., Sergeeva T.I., Novoselova G.N.
PubMed Id: 6258318
Year: 1981  Volume: 27  Issue: 1  Pages: 129-132
Teta-hemolysine was purified from Cl. perfringens strain BP6K 28 as follows: reprecipitation in the isoelectric point of the enzyme with 2 N H2SO4 containing 15% NaCl, DEAE cellulose chromatography, gel filtration on Sephadex G-100-G75 or Bio Gel P-60--P-100, rechromatography on DEAE-Sephadex A-50 and affinity chromatography. The biological activity of homogenous teta-hemolysine, estimated by complete hemolysis of human erythrocytes, exceeded 100, 000 theta E over mg protein but the enzyme was highly labile. Molecular weight of the enzyme was 53,000 daltons.
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Dolgikh, M. S., Blagoveshchenskiĭ, V. A., Sergeeva, T. I., Novoselova, G. N. (1981). Isolation of the theta-hemolysin of Cl. perfringens. Voprosy Meditsinskoi Khimii, 27(1), 129-132.
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