VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Effect on freezing on hydroxylation in isolated hepatocytes

   
Kudokotseva E.V., Gordienko A.D., Belous A.M.
PubMed Id: 7281576
Year: 1981  Volume: 27  Issue: 3  Pages: 356-359
Rapid freezing of hepatocytes down to--196 degrees was accompanied by a distinct impairment in microsomal oxidation and by complete uncoupling of respiration and oxidative phosphorylation in mitochondria. In the course of multistep freezing activity of the microsomal oxidation system constituted 70.3% with ethylene glycol (EG) and 78% - with dimethyl sulfoxide (DMSO), while the functional activity of mitochondria was maintained by 30% and 40% if EG DMSO were used, respectively.
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Kudokotseva, E. V., Gordienko, A. D., Belous, A. M. (1981). Effect on freezing on hydroxylation in isolated hepatocytes. Voprosy Meditsinskoi Khimii, 27(3), 356-359.
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