VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Several approaches to purifying rat liver G2-chalone

   
Rodionov V.M., Kuz'micheva N.A., Pospelova A.V., Pleskova M.V.
PubMed Id: 6170167
Year: 1981  Volume: 27  Issue: 4  Pages: 569-572
An attempt was made to isolate the liver tissue chalone blocking the transition of hepatocytes from G2 period of the cell cycle into mitosis/g2-chalone/. The purification was carried out using gel filtration through Sephadex G-75 and hydroxyapatite chromatography as well as by means of ultrafiltration through Diaflo membranes XM-300, XM-100 and PM-10. The biologically active preparation, obtained by these two procedures, was purified 100-fold. Molecular mass of G2 chalone was apparently less then 10,000; it penetrated through PM-10 membrane. The chalone appears to form complexes with high molecular carriers.
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Rodionov, V. M., Kuz'micheva, N. A., Pospelova, A. V., Pleskova, M. V. (1981). Several approaches to purifying rat liver G2-chalone. Voprosy Meditsinskoi Khimii, 27(4), 569-572.
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