VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Emission spectroscopic determination of the 15N in the ammonia and amino acids isolated from biological material

   
Pisarenko O.I., Minkovskiĭ E.B., Studneva I.M., Ivanov V.E.
PubMed Id: 7314591
Year: 1981  Volume: 27  Issue: 5  Pages: 710-715
A procedure for preparation of samples is described for emission-spectroscopic estimation of 15N in ammonia, glutamic aspartic acids, leucine and glycine using 15N-analyzer NOI-5. The assay involved separation of ammonia and amino acids by means of ion exchange chromatography, degradation of amino acids to ammonia its isolation by microdiffusion from buffers and subsequent reduction of ammonia to nitrogen. Amount of nitrogen in the samples was about 20 mg. The procedure enabled to estimate the content of 15N in ammonia and amino acids of physiological fluids.
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Pisarenko, O. I., Minkovskiĭ, E. B., Studneva, I. M., Ivanov, V. E. (1981). Emission spectroscopic determination of the 15N in the ammonia and amino acids isolated from biological material. Voprosy Meditsinskoi Khimii, 27(5), 710-715.
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