VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Micromethod for the fluorometric determination of the polyamine and putrescine content in animal tissues by thin-layer chromatography on Silufol UV-254 plates

   
Siatkin S.P., Berezov T.T.
PubMed Id: 7336662
Year: 1981  Volume: 27  Issue: 6  Pages: 848-851
A microquantitative, simple, rapid, highly sensitive procedure is described for estimation of polyamines and putrescine in animal tissues. Content of these substances was studied in several rapidly proliferating tissues. Statistically distinct increase in content of putrescine and polyamines was found in regenerating, malignant rat liver tissue and in hepatomas G-27 and G-46. The mean error of the assay was about 3%, its sensitivity was +/- 0.1 nmol per 1 mg of protein, reproducibility was close to 95-100%.
Download PDF:  
Citation:

Siatkin, S. P., Berezov, T. T. (1981). Micromethod for the fluorometric determination of the polyamine and putrescine content in animal tissues by thin-layer chromatography on Silufol UV-254 plates. Voprosy Meditsinskoi Khimii, 27(6), 848-851.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)