VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Reaction between 125I-collagen and growing fibronectin polypeptides of chick embryo polyribosomes

   
Zlatopol'skii A.D., Belkin V.M., Zykova T.A., Mazurov V.I.
PubMed Id: 7080470
Year: 1982  Volume: 28  Issue: 2  Pages: 108-114
Polyribosomes from chicken embryos and 125I-collagen were shown to form complexes, which might be isolated from free 125I-collagen by means of centrifugation. The fraction of rapidly sedimenting polyribosomes was the most active in formation of complexes with 125I-collagen. The complexes ribosomes-125I-collagen appeared after destruction of polyribosomes-125I-collagen complexes by means of RNAase. Treatment of the complexes with puromycine led to liberation of about 40% of 125I-collagen. Antibodies against fibronectin inhibited the complex formation by about 30%. The data obtained suggest that the growing fibronectin polypeptides participate in formation of at least 30% of the complexes.
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Zlatopol'skii, A. D., Belkin, V. M., Zykova, T. A., Mazurov, V. I. (1982). Reaction between 125I-collagen and growing fibronectin polypeptides of chick embryo polyribosomes. Voprosy Meditsinskoi Khimii, 28(2), 108-114.
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