VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Chromatographic method for quantitative determination of cordycepin in rat brain tissue after intraperitoneal injection of the antibiotic

   
Musaev N.I., Khasigov P.Z., Kuznetsov D.A.
PubMed Id: 6603710
Year: 1983  Volume: 29  Issue: 3  Pages: 134-136
A procedure is developed for quantitative estimation of intraperitoneally administered unlabelled cordycepin in acid soluble pool isolated from rat brain tissue. The fraction containing 2'- and 3'-deoxyriboadenosine (cordycepin) in acid soluble pool of rat brain was isolated after consecutive chromatography on columns of Dowex 1 X 8, Dihydroxyboryl-SP 500 and Sephasorb-HP. At the same time, 2'-deoxyriboadenosine was isolated from the acid soluble pool fraction of control animals. Content of the antibiotic in brain tissue was estimated by its difference in these two fractions (control/experiment).
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Musaev, N. I., Khasigov, P. Z., Kuznetsov, D. A. (1983). Chromatographic method for quantitative determination of cordycepin in rat brain tissue after intraperitoneal injection of the antibiotic. Voprosy Meditsinskoi Khimii, 29(3), 134-136.
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