VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

A simple and effective step in the purification of bovine blood fibronectin

   
Zykova T.A., Zlatopol'skii A.D., Mazurov V.I.
PubMed Id: 6649521
Year: 1983  Volume: 29  Issue: 5  Pages: 114-117
Preparations of fibronectin from bovine blood serum, obtained by means of affinity chromatography on collagen-Sepharose, contained immunoglobulins and other proteins, concentration of which constituted 48 +/- 5%. Differential salting out of fibronectin and other non-fibronectin proteins, using 0.8-2.0 M ammonium sulfate at pH 5.0, demonstrated that precipitation of fibronectin occurred more effectively as compared with non-fibronectin proteins at all the salt concentrations studied. If 0.8 M or 1.0 M ammonium sulfate concentrations were used, the fibronectin preparations contained less than 10% of other proteins and fibronectin loss was about 20%. Salting out of fibronectin is an effective additional step of its purification.
Download PDF:  
Citation:

Zykova, T. A., Zlatopol'skii, A. D., Mazurov, V. I. (1983). A simple and effective step in the purification of bovine blood fibronectin. Voprosy Meditsinskoi Khimii, 29(5), 114-117.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)
◄        ►