VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

A simple and effective step in the purification of bovine blood fibronectin

   
Zykova T.A., Zlatopol'skii A.D., Mazurov V.I.
PubMed Id: 6649521
Year: 1983  Volume: 29  Issue: 5  Pages: 114-117
Preparations of fibronectin from bovine blood serum, obtained by means of affinity chromatography on collagen-Sepharose, contained immunoglobulins and other proteins, concentration of which constituted 48 +/- 5%. Differential salting out of fibronectin and other non-fibronectin proteins, using 0.8-2.0 M ammonium sulfate at pH 5.0, demonstrated that precipitation of fibronectin occurred more effectively as compared with non-fibronectin proteins at all the salt concentrations studied. If 0.8 M or 1.0 M ammonium sulfate concentrations were used, the fibronectin preparations contained less than 10% of other proteins and fibronectin loss was about 20%. Salting out of fibronectin is an effective additional step of its purification.
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Zykova T.A., Zlatopol'skii A.D., Mazurov V.I. (1983) Voprosy meditsinskoi khimii, 29(5), 114-117.
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