VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Radiometric method of determining ATP: D-pantothenate-4'-phosphotransferase activity

   
Khomich T.I., Voskoboev A.I., Chernikevich I.P., Moĭseenok A.G.
PubMed Id: 6324484
Year: 1984  Volume: 30  Issue: 1  Pages: 131-132
A radiometric procedure is developed for estimation of pantothenate kinase (EC 2.7.1.33) activity in various preparations of rat liver tissue; sodium 14C-D-pantothenate was used as a substrate and the reaction end product 4'-phosphopantothenic acid was measured. Optimal separation of the substrate and the end product was achieved by means of chromatography on DEAE-Sephadex A-25. 4'-phosphopantothenic acid was eluted from the column by 0.4 N HCl thus avoiding the label dilution and possible quenching of scintillation.
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Citation:

Khomich, T. I., Voskoboev, A. I., Chernikevich, I. P., Moĭseenok, A. G. (1984). Radiometric method of determining ATP: D-pantothenate-4'-phosphotransferase activity. Voprosy Meditsinskoi Khimii, 30(1), 131-132.
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