VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Spectrophotometric micromethod to determine L-lysine-alpha-oxidase activity

   
Smirnova I.P., Siatkin S.P., Berezov T.T.
PubMed Id: 6538725
Year: 1984  Volume: 30  Issue: 1  Pages: 133-136
A spectrophotometric micromethod is developed for estimation of L-lysine-alpha-oxidase. The method is based on measurement of hydrogen peroxide formed in enzymatic oxidation of L-lysine. Optimal conditions were developed for estimation of the enzymatic activity in the extracts of Trichoderma sp. The saturating concentration of L-lysine was 10 mM. Km values for L- and DL-lysines constituted 3.0 . 10(-3) M and 6.4 . 10(-4) M, respectively. The reaction proceeded without a latent phase and H2O2 formation versus time plot had a linear shape within 20 min. The enzyme optimal activity was found at pH 5.8-6.0. The best buffer, required for lysine oxidation in the reaction, proved to be phosphate, but not succinate, borate or glycine buffers. The method described was highly sensitive and reproducible.
Download PDF:  
Citation:

Smirnova, I. P., Siatkin, S. P., Berezov, T. T. (1984). Spectrophotometric micromethod to determine L-lysine-alpha-oxidase activity. Voprosy Meditsinskoi Khimii, 30(1), 133-136.
References
 1984 (vol 30)
 1983 (vol 29)
 1982 (vol 28)
 1981 (vol 27)
 1980 (vol 26)
 1979 (vol 25)
 1978 (vol 24)
 1977 (vol 23)
 1976 (vol 22)
 1975 (vol 21)
 1974 (vol 20)
 1973 (vol 19)
 1972 (vol 18)
 1971 (vol 17)
 1970 (vol 16)
 1969 (vol 15)
 1968 (vol 14)
 1967 (vol 13)
 1966 (vol 12)
 1965 (vol 11)
◄        ►