VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

The use of immunological method for the determination of acid alpha-glucosidase activity in human muscles

   
Kreslavskaia E.E., Chibisov I.V., Antipova R.I., Narycheva I.A.
PubMed Id: 6433557
Year: 1984  Volume: 30  Issue: 3  Pages: 128-131
Homogeneous preparation of acid alpha-glucosidase, obtained from human placenta, was used for immunization of rabbits and production of specific antiserum. Immunological procedure was developed for estimation of acid alpha-glucosidase activity from human muscles in presence of the enzyme neutral form. Activity of acid alpha-glucosidase was measured in 20 patients with various types of nervous system impairments. Total activity of alpha-glucosidase in muscles of the patients was 11.4 +/- 0.7 nM/min/g of the tissue and activity of the acid form constituted about 84.8 +/- 1.7% of the total activity.
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Kreslavskaia E.E., Chibisov I.V., Antipova R.I., Narycheva I.A. (1984) Voprosy meditsinskoi khimii, 30(3), 128-131.
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