VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Effect of the conditions of isolation of free and bound ligands on the accuracy of the radioimmunoassay of steroids

   
Korneva I.A., Chaĭkovskiĭ V.S.
PubMed Id: 6506578
Year: 1984  Volume: 30  Issue: 4  Pages: 122-125
Testosterone radioimmunoassay (RIA) was used to analyse the factors which influenced the precision and accuracy of steroid RIA. Selection of optimal parameters (concentrations of activated charcoal and of the radioactive steroid) was necessary in order to reduce the influence of non-specific binding and error of fractionation on the RIA results. The scheme for estimation of the optimal values of these parameters is described.
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Korneva, I. A., Chaĭkovskiĭ, V. S. (1984). Effect of the conditions of isolation of free and bound ligands on the accuracy of the radioimmunoassay of steroids. Voprosy Meditsinskoi Khimii, 30(4), 122-125.
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